il 34 levels  (R&D Systems)

Journal: Journal of Periodontal & Implant Science

Article Title: Gingival crevicular fluid CSF-1 and IL-34 levels in patients with stage III grade C periodontitis and uncontrolled type 2 diabetes mellitus

doi: 10.5051/jpis.2106260313

Figure Lengend Snippet: Results of logistic regression analysis on CSF-1 and IL-34 levels

Article Snippet: CSF-1 and IL-34 levels in the GCF were calculated using enzyme-linked immunosorbent assay (ELISA) kits (Human CSF-1 ELISA kit and Human IL-34 ELISA kit; Elabscience, Houston, TX, USA) according to the manufacturer’s guidelines.

Techniques:

Journal: Journal of Clinical Laboratory Analysis

Article Title: IL‐34 is a potential biomarker for the treatment of papillary thyroid cancer

doi: 10.1002/jcla.23335

Figure Lengend Snippet: Protein expression of IL‐34 from human PTC samples. A, Western blot analysis of IL‐34 expression in PTC tissues, their adjacent noncancerous tissues, and normal controls. B, The ratio of IL‐34/GAPDH was determined to give a mean net density. C, Real‐time PCR analysis of IL‐34 expression in PTC tissues, their adjacent noncancerous tissues, and normal controls. Data were presented as the means ± standard error. ** P < .01 was considered significant

Article Snippet: Serum IL‐34 levels were analyzed by ELISA assay (R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction

Journal: Journal of Clinical Laboratory Analysis

Article Title: IL‐34 is a potential biomarker for the treatment of papillary thyroid cancer

doi: 10.1002/jcla.23335

Figure Lengend Snippet: IL‐34 promotes proliferation on PTC cells. Cell proliferation was determined via the CCK‐8 assay to examine the proliferation of TPC‐1 (A) and K1 cells (B) in accordance to transfected with si‐NC or si‐IL‐34. Cell proliferation in TPC‐1 (C) and K1 cells (D) which were infected with lenti‐vector or lenti‐IL‐34. Data were presented as the means ± standard error. ** P < .01 was considered significant

Article Snippet: Serum IL‐34 levels were analyzed by ELISA assay (R&D Systems, Minneapolis, MN, USA).

Techniques: CCK-8 Assay, Transfection, Infection, Plasmid Preparation

Journal: Journal of Clinical Laboratory Analysis

Article Title: IL‐34 is a potential biomarker for the treatment of papillary thyroid cancer

doi: 10.1002/jcla.23335

Figure Lengend Snippet: IL‐34 inhibits apoptotic rates on PTC cells. Cell apoptosis was determined by flow cytometry after transfected with si‐IL‐34 or si‐NC in TPC‐1 (A) and K1 cells (B). Cell apoptosis in TPC‐1 (C) and K1 cells (D) which were infected with lenti‐vector or lenti‐IL‐34. The apoptotic rates were shown. Data were presented as the means ± standard error. ** P < .01 was considered significant

Article Snippet: Serum IL‐34 levels were analyzed by ELISA assay (R&D Systems, Minneapolis, MN, USA).

Techniques: Flow Cytometry, Transfection, Infection, Plasmid Preparation

Journal: Journal of Clinical Laboratory Analysis

Article Title: IL‐34 is a potential biomarker for the treatment of papillary thyroid cancer

doi: 10.1002/jcla.23335

Figure Lengend Snippet: IL‐34 promotes the invasion via on PTC cells. Two PTC cell lines were not only transfected with si‐IL‐34 or si‐NC were also infected with lenti‐vector or lenti‐IL‐34 for 48 h. A, The protein expression levels of E‐cadherin, N‐cadherin, and Vimentin were measured by Western blotting in PTC cells. The ratios of EMT biomarkers when transfected with si‐IL‐34 or si‐NC were determined to give a mean net density in TPC‐1 (B) and K1 cells (C). The ratios of EMT biomarkers when infected with lenti‐vector or lenti‐IL‐34 were determined to give a mean net density in TPC‐1 (D) and K1 cells (E). Data were presented as the means ± standard error. ** P < .01 was considered significant

Article Snippet: Serum IL‐34 levels were analyzed by ELISA assay (R&D Systems, Minneapolis, MN, USA).

Techniques: Transfection, Infection, Plasmid Preparation, Expressing, Western Blot

Journal: Journal of Clinical Laboratory Analysis

Article Title: IL‐34 is a potential biomarker for the treatment of papillary thyroid cancer

doi: 10.1002/jcla.23335

Figure Lengend Snippet: IL‐34 promotes the invasion and activates the ERK signaling pathway on PTC cells. Two PTC cell lines were transfected with si‐IL‐34 or si‐NC were also infected with lenti‐vector or lenti‐IL‐34 for 48 h. A, The protein expression levels of p‐ERK and ERK were measured by Western blotting in PTC cells. The ratios of ERK and p‐ERK when transfected with si‐IL‐34 or si‐NC were determined to give a mean net density in TPC‐1 (B) and K1 cells (C). The ratios of ERK and p‐ERK when infected with lenti‐vector or lenti‐IL‐34 were determined to give a mean net density inTPC‐1 (D) and K1 cells (E). Data were presented as the means ± standard error. ** P < .01 was considered significant

Article Snippet: Serum IL‐34 levels were analyzed by ELISA assay (R&D Systems, Minneapolis, MN, USA).

Techniques: Transfection, Infection, Plasmid Preparation, Expressing, Western Blot